White blood cell differential count of maturation stages in bone marrow smear using dual-stage convolutional neural networks
نویسندگان
چکیده
The white blood cell differential count of the bone marrow provides information concerning the distribution of immature and mature cells within maturation stages. The results of such examinations are important for the diagnosis of various diseases and for follow-up care after chemotherapy. However, manual, labor-intensive methods to determine the differential count lead to inter- and intra-variations among the results obtained by hematologists. Therefore, an automated system to conduct the white blood cell differential count is highly desirable, but several difficulties hinder progress. There are variations in the white blood cells of each maturation stage, small inter-class differences within each stage, and variations in images because of the different acquisition and staining processes. Moreover, a large number of classes need to be classified for bone marrow smear analysis, and the high density of touching cells in bone marrow smears renders difficult the segmentation of single cells, which is crucial to traditional image processing and machine learning. Few studies have attempted to discriminate bone marrow cells, and even these have either discriminated only a few classes or yielded insufficient performance. In this study, we propose an automated white blood cell differential counting system from bone marrow smear images using a dual-stage convolutional neural network (CNN). A total of 2,174 patch images were collected for training and testing. The dual-stage CNN classified images into 10 classes of the myeloid and erythroid maturation series, and achieved an accuracy of 97.06%, a precision of 97.13%, a recall of 97.06%, and an F-1 score of 97.1%. The proposed method not only showed high classification performance, but also successfully classified raw images without single cell segmentation and manual feature extraction by implementing CNN. Moreover, it demonstrated rotation and location invariance. These results highlight the promise of the proposed method as an automated white blood cell differential count system.
منابع مشابه
Acute promyelocytic leukemia?
Briefly, the important clinical findings were as follows: A 10 year old white girl, acutely ill, febrile and showing disseminated skin purpura. There was no visceromegaly. The disease had apparently started 3 weeks before, with epistaxis and other purpuric manifestations. Laboratory findings showed severe anemia and thrombocytopenia with a leukocyte count of 3500 per cu. mm. The differential co...
متن کاملCoexistent BCR-ABL1 and JAK2 V617F: converting CML dwarves to ET staghorns with imatinib therapy.
A 67-year-old man was noted to have new-onset leukocytosis (white blood cell [WBC] count, 13.1 3 10 /mL) and thrombocytosis (platelets [PLT], 670 000/mL). The peripheral blood smear revealed granulocytes at all stages of maturation. A bone marrow (BM) biopsy revealed hypercellularity with an elevated M:E ratio. Megakaryocytes were predominantly small and hypolobated (panel A). Molecular studies...
متن کاملThe importance of bone marrow biopsy in the staging of patients with lymphosarcoma.
The frequency of bone marrow involvement in patients with lymphosarcoma (LSA) and the particular value of the marrow biopsy, as compared with the aspiration smear technique, in the initial staging of this disease were investigated. Certain physical findings and laboratory studies were analyzed to determine whether the presence of marrow invasion could be predicted. Of 75 patients, 47 (63#{176}/...
متن کاملSea-blue-colored histiocytes associated with bone marrow granulomas.
A 77-year-old man was admitted for evaluation of pancytopenia. His blood examination revealed hemoglobin 9.7 g/dL, platelet count 149 3 10/L, and white blood cell count 3.2 3 10/L with normal differential. Peripheral blood smear showed red blood cells with anisocytosis, microcytes, and teardrop-shaped cells. There was mild leukopenia with relative monocytosis and normal platelets. Bone marrow b...
متن کاملUnusual ophthalmic manifestation in chronic myeloid leukemia: A case report
Background: Chronic myeloid leukemia (CML) is a myeloproliferative hematopoietic malignancy with a heterogeneous proliferation of hematopoietic cells in the bone marrow. The ocular manifestations are rare symptoms of CML. In this case report, a CML patient with retinal hemorrhage is reported as an uncommon symptom. Case presentation: A 35-year-old man was referred due to decreased right eye vi...
متن کامل